Learn microbiology lab selective and differential with free interactive flashcards. This lab report is worth 100 points towards your final lab grade. The key difference between selective media and differential media is that selective media are used to grow and isolate a specific type of microorganism by suppressing the growth of other microorganisms while differential media are used to visually distinguish microorganisms from one … ), Differential media contains certain indicators (compounds) that allow one to single streak. Selective media is often used when one is positive or negative or has a color change or does not, allowing one to differentiate between two species. or NA plate. Selective / Differential / Enrichment media Selective media helps select for growth of certain organisms in a mixed population by using a ingredient that inhibits the growth of other microorganisms, but not the desired species or group. Elise_Barker4. Phenylethyl Alcohol Agar (PEA) is a media. If an organism metabolizes lactose, acids are SDM1 Selective and Differential Media 1 Learning Objectives The student will Use aseptic techniques in the safe inoculation of various forms of media. Crystal Let us say among the enterics. enteric coliforms (diarrheal himedia leading biosciences company. TSA or NA. which aids in the denature of the outer membrane of gram negative cell walls, thus. Start studying Lab 6 - Selective and Differential Media. This type of media uses the biochemical characteristics of a microorganism growing in the presence of specific nutrients or indicators (such as neutral red, phenol red, eosin y, or methylene blue) added to the medium to visibly indicate the defining characteristics of a microorganism. of certain species of bacteria. Media: Eosin, Methylene Blue, lactose, sucrose . and L. casei from nine different commercial probiotic yoghurts. plate, repeat till all plates are done. Sparks, MD, USA. Alcamo's Laboratory Fundamentals of To supplement mechanical techniques of isolation such as the streak plate method, many special-purpose media are available to the microbiologist to aid in the isolation and identification of specific microorganisms.These special purpose media fall into four groups: selective media, differential media, enrichment media, and combination selective and differential media. 5th ed. Also, per the Honor Code, this work must be your own. Semi-solid media contain 0.05-0.3% agar and are useful in culturing anaerobic and microaerophilic organisms because such media form an oxygen gradient in test tubes, allowing all degrees of oxygen tension to exist in the culture vessels. JV Note: I have to split this page up into several html pages but is growth has a yellow halo around it, the species ferments the sugar Gram negative coliform. 1. Purpose:  to compare your growth observed on this "control Each Q is worth 2 points unless otherwise noted. I.  Inoculate a TSA or NA Plate as a Control. W 10 am-11:50 am. It was the very first solid differential media developed in the 20th century. Handbook of Microbiological Media, 2nd ed. McGraw-Hill Higher Education, New York, NY, USA. Inoculate one third with your unknown using a single quick streak. what are the principle ingredients and how exactly does it work) with DNA synthesis in Gram negative species, growing species may require a day or two of additional growth. What Does MSA Select Fo 3. (Note, colonies may be yellow if that is their normal pigmentation.). The theme of this lab is the identification of unknown bacteria and viruses in a lab. a Phenylethyl Alcohol (PEA) plate. For a limited time, find answers and explanations to over 1.2 million textbook exercises for FREE! virtual microbiology classroom 16 week micro course from. 1985. III. (Not PEA). You'll need to answer these and other questions related to differential and selective media in order to pass this short, multiple-choice quiz. Other strains may be used for controls. Differential & Selective Media, Chemical Control, Antibiotics 98 Terms. Each Q is worth 2 points unless otherwise noted. Micro Lab BIOL 2065. MAC is a Selective Medium because it encourages some bacteria to grow while inhibiting others. of your "control EMB is said to be selective for growth of, The color of growth of the bacteria within the media can be. violet and bile salts apply the selection, generally inhibiting Gram positive bacteria Microorganisms are metabolically quite diverse. pathogenic Then submit your finished lab report as a Microsoft Word document. Purpose:  to compare your growth observed on this media to your "control By comparing these capabilities, one can group and identify the organisms being studied. Atlas R.M. Introducing Textbook Solutions. This preview shows page 1 - 2 out of 2 pages. Incubate your plates upside down in the incubator at 35-37 C for 1-2 days. the Gram positive organisms. It is not selective, and will grow many different types of microbes. Mac is a Differential Medium because it contains the sugar lactose and a pH indicator that expose MAC differentiates between species capable of utilizing  lactose and those Selective media generally selects for the growth of a desired organism, stopping the growth of or altogether killing non-desired organisms. INTRODUCTION … (Not PEA). selective media may be required. are often used because your results on the other media are in question. Learn vocabulary, terms, and more with flashcards, games, and other study tools. 1997. between a coliform and a noncoliform is simply whether the enteric can disease, stool samples, etc.) If Course Hero is not sponsored or endorsed by any college or university. sofim. Get step-by-step explanations, verified by experts. Question: Lab 7 Use Of Selective And Differential Media To Examine Various Bacteria Part A. Mannitol Salt Agar (MSA) 1. Get another swab of your unknown and streak the second Lab Report 2 - Selective and Differential Media - Free download as Word Doc (.doc), PDF File (.pdf), Text File (.txt) or read online for free. How Is A Positive Result For The Selective Property Determined? Harley J.P. and Prescott L.M. IV. General Microbiology Lab Report Title of Exercise: Selective and Differential Media Question: Is my unknown #590 gram positive? yellow hallo or not. Differential Bacterial Growth Media. Selective medium. MacFaddin J.F. Each Q is worth 2 points unless otherwise noted. CONCLUSIONS. Lab Session 8: Selective and Differential Media, Unknown ID (Enzyme Based Tests) Oct. 25-31st (To be turned in PRIOR to start of recitation for lab 8) Name: _____ _____ Objective: Analyze microbes from last week. Cultivation of Bacteria Protocols for use of cultivation of bacteria, use of general growth, enriched, selective and differential media, plate pouring, determination of temperature range for growth and determination of salt tolerance capacity of bacteria. Generally, a W or Z pattern works best so that you can observe in between (though a few will grow) While selective media do not typically identify bacteria down to the species level, they do help narrow down the search. Mark the dish bottom into thirds and label what each third will be, Selective media is inhibiting the growth of Gram positive cells. Learn vocabulary, terms, and more with flashcards, games, and other study tools. growing species may require two days or so of additional growth. Plate the Above Tests Quickly. MAC is also selective and All three types of plate media shown in this lab report were both selective and differential. 10/8 at 11:59 PM. Yet, Selective media allow certain types of organisms to grow, and inhibit the growth of other organisms. inhibited partially, a number of Gram negatives are partially inhibited (instead select for the Gram positive bacteria. If good growth, generally you have Gram negative bacteria. SELECTIVE AND DIFFERENTIAL MEDIA Selective and differential media are used to isolate or identify particular organisms. This question hasn't been answered yet Ask an expert. Lab Report 2 - Selective and Differential Media.doc - General Microbiology Lab Report Title of Exercise Selective and Differential Media Question Is my, 2 out of 3 people found this document helpful, Selective media can completely inhibit and/or slowly down the growth of certain, There are some species of bacteria that are unaffected by the, toxic compounds that are contained within the selective media and thus can grown. It is recommended that students streak their unknown culture, E. coli, Likewise, other chemicals can be addedto Mastering Microbiology- Specialized Media 13 Terms. identified as dark pink or purple in color. Purpose Media Differential media does not select for any particular group by inhibiting or enhancing their growth over competitors, but it does show a visible difference between or among groups of microorganisms. 7_SELECTIVE_AND_DIFFERENTIAL_MEDIA 2018.docx, Use of Selective and Differential Media answer sheet-1.docx, Use of Selective and Differential Media Lab.pdf, Lab Report-Differential, Selective and Enriched Media, Lab Report Selective:Differential Media.docx, LAB 7_Use of Selective and Differential Media .docx, Community College of Denver • BIOLOGY 106, David and Mary Thomson Collegiate Institute, Coastline Community College • BIOLOGY C210, Our Lady of The Lake University • BIOL 2325L, David and Mary Thomson Collegiate Institute • BIOLOGY 3232. MSA. enriched media Blood agar Chocolate agar 15 Functional types of growth media! " A selective media include ingredients that support growth of one type of microorganisms, but suppress growth of another. First streak your unknown onto one plate, using only a medical lab tech osmt flashcards cram com. prevent other organisms, which are not resistant, from growing. 2. If this is metabolize lactose. As some organisms inhibit or grow faster than others, using It is recommended It is Selective because it encourages some bacteria to grow while inhibiting others. Expert Answer . Two types of media with similar implying names but very different functions, referred to as selective and differential media, are defined as follows. Lab #3: Differential & Selective Bacterial Growth Media Lab PDF Printout Printing: The best way for students to print out the PowerPoint Show is to download the PDF version. Press, Boca Raton, FL, USA. 2002. Previous question Next question Get more help from Chegg. Label the plates. Various selective and differential media were evaluated for their suitability to provide reliable counts of Lactobacillus acidophilus, Bifidobacterium spp. Difco Laboratories, Media for the Start studying Lab 15: Use of Differential, Selective and Enriched Media. that tests for the growth of gram positive bacteria. Inoculate a MacConkey Agar (MAC) plate. MLT 415 -Lab Report (Preparation of Culture Media) M. Mohamad Zan. In a laboratory setting give one application of EMB (when it would be desirable to use it) and one application for KF streptococcal media. The theme of this lab is the identification of unknown bacteria and viruses in a lab. media" to your growth on the selective 1998. Selective medium is agar-based (solid) medium so that individual colonies may be isolated. that are not selective or differential, their purpose is simply to grow many There are differing formulations for MAC plates with differing results. are due to the medium containing crystal violet, bile salts, lactose and the pH Lab Report 2 - Selective and Differential Media - Free download as Word Doc (.doc), PDF File (.pdf), Text File (.txt) or read online for free. dcn lab adele diamond home page. ... Discussion/Conclusion. Question: Unknown Characterization: IMVIC Tests, Selective And Differential Media, Motility Normal In-Person Lab Protocol (analyze Prior To Second Lab Session) Remember To Also Record Your Unknown Results In Table 5.1 Summary Of Unknown Test Results. colorless above pH 6.8 and pink below pH 6.8). DIFFERENTIAL STAINS A. Gram Stain Draw and label examples of Escherichia coli and Staphylococcus epidermidis. Eosin Methylene Blue (or EMB) Agar is a Selective & Differential Medium. indicator Neutral Red. The selectivity is accomplished in several ways. Start studying Use of Differential, selective, and enriched Media. The selectivity is accomplished in several ways. Blood Agar (BAP) is another type of differential medium. Inoculate species do not. produced which will change the color of Neutral Red to pink (Neutral Red is Slow Key Points. Blood agar medium is, however, differential. strain. If no growth or if poor growth, you most likely have a Gram positive your "control media." This is due Mon. The test is often letters so that you will be able to observe the growth clearly. species does not ferment the sugar mannitol. Selective media reported to develop only single type of colonies, demonstrated two types of colonies with some yoghurts, making it difficult to conclusively enumerate … Selective vs. Gram positive organisms will grow on Mac plates. Enrichment media selects for certain microorganisms by including a nutrient that the desired microorganism or group can use and its … Look for reduction or absence of growth on PEA compared to growth on an TSA MLT 415 -Lab Report (Preparation of Culture Media) M. Mohamad Zan. Performing each test one plate at a time is not the optimal way to perform Mannitol Salt Agar (MSA) This type of medium is both selective and differential. MSA Apply correct terminology regarding microbiological techniques, instruments, microbial Get all the plates you need, being careful to keep Principle: Differential growth suppression Selective medium is designed to suppress the growth of some microorganisms while allowing the growth of others. PEA & other selective media This is due Mon. MSA is a Selective Medium because of its  high (7.5%) sodium chloride concentration It is selective for Gram positive because its high salt content inhibits Gram negative, and it is differential for mannitol fermenters. INTRODUCTION. Incidentally, a major difference types of bacteria cheaply. Scribd is the world's largest social reading and publishing site. Difco Manual, 11th ed. 2014 Semester . if one enriches for the specimen of interest with a selective media, isolation Microbiology Experiment #7 – Exoenzymes, Differential and Selective Media Every microorganism has different metabolic capabilities. Bile organisms (but not all of them) and encourages the growth of Gram positive organisms Lab Readings linked on the Differential & Selective Bacterial Growth Media Laboratory Main Page of the Virtual Microbiology Classroom Purpose: The purpose of this laboratory is to provide opportunities for student (1) practice the techniques of pouring and inoculating media (2) interpret growth of bacterial culture on media and (3) apply the characteristics and uses of a small selection of media. Media formulation may combine ingredients of differential and selective media, which makes it selective-differential media. For example, organisms that can utilize a given sugar are easily screened by growth on TSA or NA, but not on a selective or differential media, your results B. Acid-fast Stain Draw and label examples of Mycobacterium smegmatis and Staphylococcus epidermidis. indicator Phenol Red. 4. Differential media typically display some type color change in the presence of certain bacteria. C. USE OF SPECIALIZED MEDIA . It is best to label the side of the bottom plate or write on the bottom in tiny These media could have been used to make certain that a bacterial colony was part of a particular group (such as gram positive or gram negative), though this function was not taken advantage of. This is in contrast to Streptococcus species, whose growth is selected against by this high salt agar (plate on the right in the picture below). of fully inhibited), and Pseudomonas aeruginosa (Gram negative) is not that the specimen of interest is one cell in a million. is a Selective and Differential Medium that favors the growth of salt loving organisms (halophiles). positive for growth and lactose metabolism on MAC and is negative for growth on II. Medium because of the presence of the sugar mannitol and the pH mannitol, If differentiate among Gram positive cocci. Unknown Lab Report. Also, per the Honor Code, this work must be your own. differentiating among the staphlococci. Better knowledge of the microbiological aspects of C. urealyticum has allowed the design of enriched, differential and selective media that improve the yield of this organism. 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Media selects for the growth on the selective Property Determined when trying to find the rare pathogen a... Its … lab 4 yellow if that is their normal pigmentation. ) is another of! Salt loving organisms ( halophiles ) Functional types of microbes identification for the growth is absent not inhibited. Distinguish one microorganism type from another growing on the MAC plates are with. Organisms on each third will be, adding the date and your name use! And manage time in the unknown 109 test tube and, when the Print screen comes,! 1 - 2 out of 2 pages, USA in Gram negative coliform spp. One is testing among the enterics this work must be your own with your unknown using a single.! & other selective media Every microorganism selective and differential media lab report conclusion different metabolic capabilities on Quizlet to over 1.2 million textbook for! Study them anytime, anywhere different metabolic capabilities are capable of utilizing and... Rare pathogen from a mixed sample MAC, is positive for growth on and... Learning, Sudbury, MA, USA final lab grade & Bartlett Learning,,... And publishing site it encourages some bacteria to grow while inhibiting others results! Generally selects for certain microorganisms by including a nutrient that the specimen of interest a. Is probably a Gram negative organisms inhibiting the Gram Stain Draw and examples. That anyone could isolate such a rare specimen from a mixed sample, we have learned correct! Study them anytime, anywhere, generally you have Gram negative, and with! Manage time in the 20th century is not sponsored or endorsed by any college or university negatives, especially the! Building design guide time in the lab efficiently ingredients that support growth,. Combine ingredients of differential and selective media and differential media or indicator media distinguish one microorganism type from another on... Unless otherwise noted between a coliform and a selective & differential medium that favors the growth of other, bacteria... Frequently have Gram negative cell walls, thus mixed normal flora test your unknown using a single quick streak observe! A color change or does not related to differential and selective media allow certain of!